A functional family of fluorescent nucleotide analogues to investigate actin dynamics and energetics.
Nat Commun
; 12(1): 548, 2021 01 22.
Article
en En
| MEDLINE
| ID: mdl-33483497
Actin polymerization provides force for vital processes of the eukaryotic cell, but our understanding of actin dynamics and energetics remains limited due to the lack of high-quality probes. Most current probes affect dynamics of actin or its interactions with actin-binding proteins (ABPs), and cannot track the bound nucleotide. Here, we identify a family of highly sensitive fluorescent nucleotide analogues structurally compatible with actin. We demonstrate that these fluorescent nucleotides bind to actin, maintain functional interactions with a number of essential ABPs, are hydrolyzed within actin filaments, and provide energy to power actin-based processes. These probes also enable monitoring actin assembly and nucleotide exchange with single-molecule microscopy and fluorescence anisotropy kinetics, therefore providing robust and highly versatile tools to study actin dynamics and functions of ABPs.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Citoesqueleto de Actina
/
Actinas
/
Proteínas de Microfilamentos
/
Proteínas Musculares
/
Nucleótidos
Límite:
Animals
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Francia