Preparing Better Samples for Cryo-Electron Microscopy: Biochemical Challenges Do Not End with Isolation and Purification.
Annu Rev Biochem
; 90: 451-474, 2021 06 20.
Article
en En
| MEDLINE
| ID: mdl-33556280
ABSTRACT
The preparation of extremely thin samples, which are required for high-resolution electron microscopy, poses extreme risk of damaging biological macromolecules due to interactions with the air-water interface. Although the rapid increase in the number of published structures initially gave little indication that this was a problem, the search for methods that substantially mitigate this hazard is now intensifying. The two main approaches under investigation are (a) immobilizing particles onto structure-friendly support films and (b) reducing the length of time during which such interactions may occur. While there is little possibility of outrunning diffusion to the interface, intentional passivation of the interface may slow the process of adsorption and denaturation. In addition, growing attention is being given to gaining more effective control of the thickness of the sample prior to vitrification.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Microscopía por Crioelectrón
/
Complejos Multiproteicos
Idioma:
En
Revista:
Annu Rev Biochem
Año:
2021
Tipo del documento:
Article