Novel Protein-oligonucleotide Conjugation Method Involving a High-affinity Capture HaloTag.
Bio Protoc
; 10(18): e3759, 2020 Sep 20.
Article
en En
| MEDLINE
| ID: mdl-33659418
ABSTRACT
Highly sensitive quantitative protein profiling can play a key role in the early diagnosis of diseases, such as autoimmune diseases and cancer. We developed a modified protein-oligonucleotide conjugation method termed HaloTag-mediated barcoding, for quantifying protein molecules at a higher sensitivity than conventional protein quantification methods. This novel and efficient conjugation method can be used to prepare HaloTag-barcoded proteins using a click chemistry-based labeling technique. Here, we describe the preparation of protein-DNA complexes and detection of protein-protein interactions which can be used in a HaloTag protein barcode assay to detect an antibody. The protocol includes procedures for preparing the ligand-oligonucleotide complex, plasmid DNA preparation for protein expression, and preparation of the protein-oligonucleotide complex. The described click reaction-based protocols simplify the conventional amine-ester reaction methods which require additional steps for chromatography purification.
Texto completo:
1
Banco de datos:
MEDLINE
Tipo de estudio:
Guideline
/
Screening_studies
Idioma:
En
Revista:
Bio Protoc
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón