Enrichment and detection method for the prognostic value of circulating tumor cells in ovarian cancer: A meta-analysis.

ABSTRACT

OBJECTIVES: Recent studies have revealed that circulating tumor cells (CTCs) might predict bad prognosis, but the results were conflicting. Sampling time, treatment, enrichment method and detection method also varied. We aimed to evaluate whether patients with CTCs in peripheral blood have bad survival outcomes with consideration of the above four aspects. METHODS: Relevant studies were searched on Pubmed, Embase and the Cochrane Library. Studies of CTCs involving survival data available were identified for a systematic review and meta-analysis. HRs and 95% CIs for PFS and OS were extracted directly or from the Kaplan-Meier survival curves by the Engauge Digitizer v4.1. Subgroup analyses were performed to evaluate the effect of sampling time, treatment, enrichment method and detection method. RESULTS: Two clinical trials and thirteen retrospective studies with a total of 1285 patients were included. CTCs significantly correlated with OS (HR = 1.77, 95%CI1.42-2.21, p < 0.00001 and PFS (HR = 1.53, 95%CI1.26-1.86, p < 0.0001). Subgroup analyses showed that CTCs were significant associated with OS in the "Pre-therapy" subgroup (HR = 1.79, 95%CI1.43-2.24, p < 0.00001), the "Surgery" group (HR = 1.82, 95%CI1.42-2.33, p < 0.00001), and the "RT-PCR"subgroup (HR = 2.29, 95%CI1.53-3.42, p < 0.0001). While for enrichment method, CTCs significantly correlated with OS in the"Physical method" subgroup (HR = 1.94, 95%CI1.21-3.09, p = 0.006) and the "Immunological method" subgroup (HR = 1.84, 95%CI1.37-2.48, p < 0.0001). CONCLUSIONS: The presence of CTCs prior to the treatment indicated worse OS and PFS and CTCs might be predictive biomarker for ovarian cancer patients . CTCs detected using RT-PCR seem to be associated with poorer OS and PFS in patients with ovarian cancer.