Application of single-cell transcriptomics to kinetoplastid research.
Parasitology
; 148(10): 1223-1236, 2021 09.
Article
en En
| MEDLINE
| ID: mdl-33678213
ABSTRACT
Kinetoplastid parasites are responsible for both human and animal diseases across the globe where they have a great impact on health and economic well-being. Many species and life cycle stages are difficult to study due to limitations in isolation and culture, as well as to their existence as heterogeneous populations in hosts and vectors. Single-cell transcriptomics (scRNA-seq) has the capacity to overcome many of these difficulties, and can be leveraged to disentangle heterogeneous populations, highlight genes crucial for propagation through the life cycle, and enable detailed analysis of hostparasite interactions. Here, we provide a review of studies that have applied scRNA-seq to protozoan parasites so far. In addition, we provide an overview of sample preparation and technology choice considerations when planning scRNA-seq experiments, as well as challenges faced when analysing the large amounts of data generated. Finally, we highlight areas of kinetoplastid research that could benefit from scRNA-seq technologies.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Kinetoplastida
/
Perfilación de la Expresión Génica
/
Análisis de la Célula Individual
Idioma:
En
Revista:
Parasitology
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido