Interaction standards for biophysics: anti-lysozyme nanobodies.
Eur Biophys J
; 50(3-4): 333-343, 2021 May.
Article
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| MEDLINE
| ID: mdl-33839878
ABSTRACT
There is a significant demand in the molecular biophysics community for robust standard samples. They are required by researchers, instrument developers and pharmaceutical companies for instrumental quality control, methodological development and in the design and validation of devices, diagnostics and instrumentation. To-date there has been no clear consensus on the need and type of standards that should be available and different research groups and instrument manufacturers use different standard systems which significantly hinders comparative analysis. One of the major objectives of the Association of Resources for Biophysical Research in Europe (ARBRE) is to establish a common set of standard samples that can be used throughout the biophysics community and instrument developers. A survey was circulated among ARBRE members to ascertain the requirements of laboratories when using standard systems and the results are documented in this article. In summary, the major requirements are protein samples which are cheap, relatively small, stable and have different binding strengths. We have developed a panel of sdAb's or 'nanobodies' against hen-egg white lysozyme with different binding strengths and suitable stability characteristics. Here we show the results of the survey, the selection procedure, validation and final selection of a panel of nanobody interaction standards.
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Banco de datos:
MEDLINE
Asunto principal:
Anticuerpos de Dominio Único
Tipo de estudio:
Guideline
Límite:
Animals
Idioma:
En
Revista:
Eur Biophys J
Asunto de la revista:
BIOFISICA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido