The vaginal microbiota composition of women undergoing assisted reproduction: a prospective cohort study.
BJOG
; 128(13): 2101-2109, 2021 Dec.
Article
en En
| MEDLINE
| ID: mdl-34053157
ABSTRACT
OBJECTIVE:
To evaluate the impact of vaginal microbiota on pregnancy outcomes in women undergoing assisted reproduction.DESIGN:
A prospective cohort study.SETTING:
A university-based assisted reproductive technology (ART) centre. POPULATION 223 women undergoing ART treatment.METHODS:
Prior to embryo transfer, vaginal samples were collected from the posterior fornix. Vaginal microbiota identification was carried out using next-generation sequencing and categorised according to the V3-V4 hypervariable region in the 16S rRNA gene region. MAIN OUTCOMEMEASURES:
ART clinical outcomes (implantation, clinical pregnancy rates and live birth rates).RESULTS:
The live birth rate in women with community state type (CST)-I (39%) was higher than that in women with CST-III (21.5%) but the difference was not statistically significant (P = 0.052). The relative abundance of Lactobacillus was lower in women who failed to become pregnant (NP group) (67.71%) than in women who became pregnant (PR group) (79.72%). However, this difference was not statistically significant (P = 0.06). In the NP group, the relative abundance of Streptococcus (7.81%) and Gardnerella (9.40%) was higher than that in the PR group (relative abundance of Streptococcus and Gardnerella was 2.28% and 5.56%, respectively). The abundance of Streptococcus was found to be statistically significantly different between the two study groups (P = 0.014). Linear discriminant analysis (LDA) further validated that Streptococcus had the highest contribution (LDA score >4.0) to the difference between these two groups.CONCLUSIONS:
Streptococcus has the highest contribution to the distinction between the PR and NP groups. TWEETABLE ABSTRACT A relatively high abundance of Streptococcus in the vaginal microbiota may be associated with a lower ART success rate.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Vagina
/
Tasa de Natalidad
/
Índice de Embarazo
/
Técnicas Reproductivas Asistidas
/
Microbiota
/
Lactobacillus
Tipo de estudio:
Etiology_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Límite:
Adult
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
BJOG
Asunto de la revista:
GINECOLOGIA
/
OBSTETRICIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Turquía