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Real-time monitoring of subcellular H2O2 distribution in Chlamydomonas reinhardtii.
Niemeyer, Justus; Scheuring, David; Oestreicher, Julian; Morgan, Bruce; Schroda, Michael.
Afiliación
  • Niemeyer J; Molekulare Biotechnologie & Systembiologie, TU Kaiserslautern, Paul-Ehrlich Straße 23, D-67663 Kaiserslautern, Germany.
  • Scheuring D; Phytopathologie, TU Kaiserslautern, Paul-Ehrlich Straße 22, D-67663 Kaiserslautern, Germany.
  • Oestreicher J; Institute of Biochemistry, Zentrum für Human und Molekularbiologie (ZHMB), Saarland University, D-66123 Saarbrücken, Germany.
  • Morgan B; Institute of Biochemistry, Zentrum für Human und Molekularbiologie (ZHMB), Saarland University, D-66123 Saarbrücken, Germany.
  • Schroda M; Molekulare Biotechnologie & Systembiologie, TU Kaiserslautern, Paul-Ehrlich Straße 23, D-67663 Kaiserslautern, Germany.
Plant Cell ; 33(9): 2935-2949, 2021 09 24.
Article en En | MEDLINE | ID: mdl-34196712
ABSTRACT
Hydrogen peroxide (H2O2) is recognized as an important signaling molecule in plants. We sought to establish a genetically encoded, fluorescent H2O2 sensor that allows H2O2 monitoring in all major subcompartments of a Chlamydomonas cell. To this end, we used the Chlamydomonas Modular Cloning toolbox to target the hypersensitive H2O2 sensor reduction-oxidation sensitive green fluorescent protein2-Tsa2ΔCR to the cytosol, nucleus, mitochondrial matrix, chloroplast stroma, thylakoid lumen, and endoplasmic reticulum (ER). The sensor was functional in all compartments, except for the ER where it was fully oxidized. Employing our novel sensors, we show that H2O2 produced by photosynthetic linear electron transport (PET) in the stroma leaks into the cytosol but only reaches other subcellular compartments if produced under nonphysiological conditions. Furthermore, in heat-stressed cells, we show that cytosolic H2O2 levels closely mirror temperature up- and downshifts and are independent from PET. Heat stress led to similar up- and downshifts of H2O2 levels in the nucleus and, more mildly, in mitochondria but not in the chloroplast. Our results thus suggest the establishment of steep intracellular H2O2 gradients under normal physiological conditions with limited diffusion into other compartments. We anticipate that these sensors will greatly facilitate future investigations of H2O2 biology in plant cells.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Chlamydomonas reinhardtii / Peróxido de Hidrógeno Idioma: En Revista: Plant Cell Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Chlamydomonas reinhardtii / Peróxido de Hidrógeno Idioma: En Revista: Plant Cell Asunto de la revista: BOTANICA Año: 2021 Tipo del documento: Article País de afiliación: Alemania