Generation and characterization of a human iPSC line SANi007-A from a patient with a heterozygous dominant mutation in ELANE.
Stem Cell Res
; 55: 102440, 2021 08.
Article
en En
| MEDLINE
| ID: mdl-34229202
ABSTRACT
Induced pluripotent stem cell (iPSC) line was generated from erythroblasts (EBLs) derived from a patient diagnosed with severe congenital neutropenia, caused by mutations in ELANE (c.614delG). Transgene-free iPSC line was generated using Sendai virus reprogramming. The iPSC line showed normal karyotype, expressed pluripotency associated genes and was capable of in vitro spontaneous differentiation towards the three germ layers. The generated iPSC line can be used to study severe congenital neutropenia and the role of neutrophil elastase protein.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Madre Pluripotentes Inducidas
Límite:
Humans
Idioma:
En
Revista:
Stem Cell Res
Año:
2021
Tipo del documento:
Article
País de afiliación:
Países Bajos