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Remote control of neural function by X-ray-induced scintillation.
Matsubara, Takanori; Yanagida, Takayuki; Kawaguchi, Noriaki; Nakano, Takashi; Yoshimoto, Junichiro; Sezaki, Maiko; Takizawa, Hitoshi; Tsunoda, Satoshi P; Horigane, Shin-Ichiro; Ueda, Shuhei; Takemoto-Kimura, Sayaka; Kandori, Hideki; Yamanaka, Akihiro; Yamashita, Takayuki.
Afiliación
  • Matsubara T; Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya, Japan.
  • Yanagida T; Department of Neural Regulation, Graduate School of Medicine, Nagoya University, Nagoya, Japan.
  • Kawaguchi N; Department of Physiology, Fujita Health University School of Medicine, Toyoake, Japan.
  • Nakano T; Nara Institute of Science and Technology, Nara, Japan.
  • Yoshimoto J; Nara Institute of Science and Technology, Nara, Japan.
  • Sezaki M; Nara Institute of Science and Technology, Nara, Japan.
  • Takizawa H; Department of Computational Biology, Fujita Health University School of Medicine, Toyoake, Japan.
  • Tsunoda SP; Nara Institute of Science and Technology, Nara, Japan.
  • Horigane SI; International Research Center for Medical Sciences, Kumamoto University, Kumamoto, Japan.
  • Ueda S; International Research Center for Medical Sciences, Kumamoto University, Kumamoto, Japan.
  • Takemoto-Kimura S; Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Nagoya, Japan.
  • Kandori H; PRESTO, Japan Science and Technology Agency, Kawaguchi, Japan.
  • Yamanaka A; Department of Neuroscience I, Research Institute of Environmental Medicine, Nagoya University, Nagoya, Japan.
  • Yamashita T; Department of Molecular/Cellular Neuroscience, Graduate School of Medicine, Nagoya University, Nagoya, Japan.
Nat Commun ; 12(1): 4478, 2021 07 22.
Article en En | MEDLINE | ID: mdl-34294698
ABSTRACT
Scintillators emit visible luminescence when irradiated with X-rays. Given the unlimited tissue penetration of X-rays, the employment of scintillators could enable remote optogenetic control of neural functions at any depth of the brain. Here we show that a yellow-emitting inorganic scintillator, Ce-doped Gd3(Al,Ga)5O12 (CeGAGG), can effectively activate red-shifted excitatory and inhibitory opsins, ChRmine and GtACR1, respectively. Using injectable CeGAGG microparticles, we successfully activated and inhibited midbrain dopamine neurons in freely moving mice by X-ray irradiation, producing bidirectional modulation of place preference behavior. CeGAGG microparticles are non-cytotoxic and biocompatible, allowing for chronic implantation. Pulsed X-ray irradiation at a clinical dose level is sufficient to elicit behavioral changes without reducing the number of radiosensitive cells in the brain and bone marrow. Thus, scintillator-mediated optogenetics enables minimally invasive, wireless control of cellular functions at any tissue depth in living animals, expanding X-ray applications to functional studies of biology and medicine.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Encéfalo Límite: Animals / Female / Humans / Male Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2021 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Encéfalo Límite: Animals / Female / Humans / Male Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2021 Tipo del documento: Article País de afiliación: Japón