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Double- to Single-Strand Transition Induces Forces and Motion in DNA Origami Nanostructures.
Gür, Fatih N; Kempter, Susanne; Schueder, Florian; Sikeler, Christoph; Urban, Maximilian J; Jungmann, Ralf; Nickels, Philipp C; Liedl, Tim.
Afiliación
  • Gür FN; Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.
  • Kempter S; Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.
  • Schueder F; Research Group Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.
  • Sikeler C; Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, New Haven, 06510, United States.
  • Urban MJ; Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.
  • Jungmann R; Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.
  • Nickels PC; Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539, Munich, Germany.
  • Liedl T; Research Group Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.
Adv Mater ; 33(37): e2101986, 2021 Sep.
Article en En | MEDLINE | ID: mdl-34337805
The design of dynamic, reconfigurable devices is crucial for the bottom-up construction of artificial biological systems. DNA can be used as an engineering material for the de-novo design of such dynamic devices. A self-assembled DNA origami switch is presented that uses the transition from double- to single-stranded DNA and vice versa to create and annihilate an entropic force that drives a reversible conformational change inside the switch. It is distinctively demonstrated that a DNA single-strand that is extended with 0.34 nm per nucleotide - the extension this very strand has in the double-stranded configuration - exerts a contractive force on its ends leading to large-scale motion. The operation of this type of switch is demonstrated via transmission electron microscopy, DNA-PAINT super-resolution microscopy and darkfield microscopy. The work illustrates the intricate and sometimes counter-intuitive forces that act in nanoscale physical systems that operate in fluids.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: ADN / Nanoestructuras Idioma: En Revista: Adv Mater Asunto de la revista: BIOFISICA / QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: ADN / Nanoestructuras Idioma: En Revista: Adv Mater Asunto de la revista: BIOFISICA / QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Alemania