Investigation of cardiac glycosides from oleander in a human induced pluripotent stem cells derived cardiomyocyte model.
Toxicol Lett
; 350: 261-266, 2021 Oct 10.
Article
en En
| MEDLINE
| ID: mdl-34371141
ABSTRACT
The ingestion of Nerium oleander and Thevetia peruviana are common causes for poisoning in Southeast Asia. All parts of the oleander shrub contain cardiac glycosides of the cardenolide type. These glycosides act via inhibition of a Na+/K+-ATPase which might cause severe arrhythmia and subsequent death in oleander-poisoned patients. The current study uses human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM) in a microelectrode array (MEA) system to assess the cardiac effects of neriifolin, oleandrin, digitoxigenin, peruvoside and thevetin A from the oleander plant. Digoxin was used as established reference compound. All tested compounds showed a corrected field potential duration (FPDc) shortening and was the lowest for 600 nM digitoxigenin with -36.9 ± 1.2 %. Next to the dose-dependent pro-arrhythmic potential, a complete beat arrest of the spontaneously beating hiPSC-CM was observed at a concentration of 300 nM for neriifolin, 600 nM for oleandrin and 1000 nM for digitoxigenin and peruvoside. Thevetin A did not cause arrhythmia up to a final concentration of 1000 nM. Thus, it was possible to establish a cardiac effect rank order of the tested substances neriifolin > oleandrin > digitoxigenin = peruvoside > digoxin > thevetin A.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Intoxicación por Plantas
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Cardenólidos
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Glicósidos Cardíacos
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Células Cultivadas
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Nerium
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Miocitos Cardíacos
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Células Madre Pluripotentes Inducidas
Límite:
Humans
Idioma:
En
Revista:
Toxicol Lett
Año:
2021
Tipo del documento:
Article
País de afiliación:
Alemania