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Preformed Ω-profile closure and kiss-and-run mediate endocytosis and diverse endocytic modes in neuroendocrine chromaffin cells.
Shin, Wonchul; Wei, Lisi; Arpino, Gianvito; Ge, Lihao; Guo, Xiaoli; Chan, Chung Yu; Hamid, Edaeni; Shupliakov, Oleg; Bleck, Christopher K E; Wu, Ling-Gang.
Afiliación
  • Shin W; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
  • Wei L; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
  • Arpino G; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
  • Ge L; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
  • Guo X; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
  • Chan CY; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
  • Hamid E; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
  • Shupliakov O; Department of Neuroscience, Karolinska Institutet, 17177 Stockholm, Sweden; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg 199034, Russia.
  • Bleck CKE; National Heart, Lung, and Blood Institute, Bethesda, MD, USA.
  • Wu LG; National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA. Electronic address: wul@ninds.nih.gov.
Neuron ; 109(19): 3119-3134.e5, 2021 10 06.
Article en En | MEDLINE | ID: mdl-34411513
Transformation of flat membrane into round vesicles is generally thought to underlie endocytosis and produce speed-, amount-, and vesicle-size-specific endocytic modes. Visualizing depolarization-induced exocytic and endocytic membrane transformation in live neuroendocrine chromaffin cells, we found that flat membrane is transformed into Λ-shaped, Ω-shaped, and O-shaped vesicles via invagination, Λ-base constriction, and Ω-pore constriction, respectively. Surprisingly, endocytic vesicle formation is predominantly from not flat-membrane-to-round-vesicle transformation but calcium-triggered and dynamin-mediated closure of (1) Ω profiles formed before depolarization and (2) fusion pores (called kiss-and-run). Varying calcium influxes control the speed, number, and vesicle size of these pore closures, resulting in speed-specific slow (more than ∼6 s), fast (less than ∼6 s), or ultrafast (<0.6 s) endocytosis, amount-specific compensatory endocytosis (endocytosis = exocytosis) or overshoot endocytosis (endocytosis > exocytosis), and size-specific bulk endocytosis. These findings reveal major membrane transformation mechanisms underlying endocytosis, diverse endocytic modes, and exocytosis-endocytosis coupling, calling for correction of the half-a-century concept that the flat-to-round transformation predominantly mediates endocytosis after physiological stimulation.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Cromafines / Endocitosis / Células Neuroendocrinas Límite: Animals Idioma: En Revista: Neuron Asunto de la revista: NEUROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Cromafines / Endocitosis / Células Neuroendocrinas Límite: Animals Idioma: En Revista: Neuron Asunto de la revista: NEUROLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos