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Assessment and Validation of Globodera pallida as a Novel In Vivo Model for Studying Alzheimer's Disease.
Althobaiti, Norah A; Menaa, Farid; Albalawi, Aishah E; Dalzell, Johnathan J; Warnock, Neil D; Mccammick, Erin M; Alsolais, Abdulellah; Alkhaibari, Abeer M; Green, Brian D.
Afiliación
  • Althobaiti NA; Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast BT9 5DL, UK.
  • Menaa F; Biology Department, College of Science and Humanities-Al Quwaiiyah, Shaqra University, Al Quwaiiyah 19257, Saudi Arabia.
  • Albalawi AE; Departments of Internal Medicine and Advanced Technologies, Fluorotronics-California Innovations Corporation, San Diego, CA 92037, USA.
  • Dalzell JJ; Biology Department, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia.
  • Warnock ND; Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast BT9 5DL, UK.
  • Mccammick EM; Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast BT9 5DL, UK.
  • Alsolais A; Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast BT9 5DL, UK.
  • Alkhaibari AM; Nursing Department, Faculty of Applied Health Science, Shaqra University, Al Dawadmi 17452, Saudi Arabia.
  • Green BD; Biology Department, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia.
Cells ; 10(9)2021 09 19.
Article en En | MEDLINE | ID: mdl-34572130
BACKGROUND: Whole transgenic or non-transgenic organism model systems allow the screening of pharmacological compounds for protective actions in Alzheimer's disease (AD). AIM: In this study, a plant parasitic nematode, Globodera pallida, which assimilates intact peptides from the external environment, was investigated as a new potential non-transgenic model system of AD. Methods: Fresh second-stage juveniles of G. pallida were used to measure their chemosensory, perform immunocytochemistry on their neurological structures, evaluate their survival rate, measure reactive oxygen species, and determine total oxidized glutathione to reduced glutathione ratio (GSSG/GSH) levels, before and after treatment with 100 µM of various amyloid beta (Aß) peptides (1-40, 1-42, 17-42, 17-40, 1-28, or 1-16). Wild-type N2 C. elegans (strain N2) was cultured on Nematode Growth Medium and directly used, as control, for chemosensory assays. RESULTS: We demonstrated that: (i) G. pallida (unlike Caenorhabditis elegans) assimilates amyloid-ß (Aß) peptides which co-localise with its neurological structures; (ii) pre-treatment with various Aß isoforms (1-40, 1-42, 17-42, 17-40, 1-28, or 1-16) impairs G. pallida's chemotaxis to differing extents; (iii) Aß peptides reduced survival, increased the production of ROS, and increased GSSG/GSH levels in this model; (iv) this unique model can distinguish differences between different treatment concentrations, durations, and modalities, displaying good sensitivity; (v) clinically approved neuroprotective agents were effective in protecting G. pallida from Aß (1-42) exposure. Taken together, the data indicate that G. pallida is an interesting in vivo model with strong potential for discovery of novel bioactive compounds with anti-AD activity.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Tylenchoidea / Animales Modificados Genéticamente / Péptidos beta-Amiloides / Especies Reactivas de Oxígeno / Estrés Oxidativo / Fármacos Neuroprotectores / Enfermedad de Alzheimer Límite: Animals Idioma: En Revista: Cells Año: 2021 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Tylenchoidea / Animales Modificados Genéticamente / Péptidos beta-Amiloides / Especies Reactivas de Oxígeno / Estrés Oxidativo / Fármacos Neuroprotectores / Enfermedad de Alzheimer Límite: Animals Idioma: En Revista: Cells Año: 2021 Tipo del documento: Article