Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model.
STAR Protoc
; 3(1): 101060, 2022 03 18.
Article
en En
| MEDLINE
| ID: mdl-35005642
ABSTRACT
Macrophage (MΦ) activation and promotion of fibrosis are critical processes in lens capsule healing after injury. Here, we detail a protocol that induces MΦ2 formation within the vitreous body of the eye. Our procedure combines the use of an intravitreal injection of a growth factor (CSF-1) and immunofluorescence to confirm the presence of MΦ2 and fibrotic tissue formation. This protocol allows assessment of the distribution of macrophages and quantification of fibrotic tissue formation/sealing within the vitreous body of mouse eyes. For complete details on the use and execution of this profile, please refer to Li et al. (2021), Gerhardt et al. (2003), Kubota et al. (2009).
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Factor Estimulante de Colonias de Macrófagos
/
Cristalino
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2022
Tipo del documento:
Article
País de afiliación:
Estados Unidos