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Reversible Near-Infrared Fluorescence Photoswitching in Aqueous Media by Diarylethene: Toward High-Accuracy Live Optical Imaging.
Jung, Hye-Youn; Kim, Boram; Jeon, Min Ho; Kim, Yoonkyung.
Afiliación
  • Jung HY; Division of Biomedical Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Korea.
  • Kim B; Division of Biomedical Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Korea.
  • Jeon MH; Division of Biomedical Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Korea.
  • Kim Y; Division of Biomedical Research, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Korea.
Small ; 18(10): e2103523, 2022 03.
Article en En | MEDLINE | ID: mdl-35023602
Fluorescence imaging is an indispensable tool in modern biological research, allowing simple and inexpensive color-coded visualizations of real-time events in living cells and animals, as well as of fixed states of ex vivo specimens. The accuracy of fluorescence imaging in living systems is, however, impeded by autofluorescence, light scattering, and limited penetration depth of light. Nevertheless, the clinical use of fluorescence imaging is expected to grow along with advances in imaging equipment, and will increasingly demand high-accuracy probes to avoid false-positive results in disease detection. To this end, a water-soluble and relatively safe diarylethene (DAE)-based reversible near-infrared (NIR) fluorescence photoswitch for living systems is prepared here. Furthermore, to facilitate excellent switching performance, the photoirradiation results obtained is compared using three different visible light sources to turn on NIR fluorescence through cycloreversion of DAE. While photoswitching using 589 nm light leads to slightly higher cell viability, fluorescence quenching efficiency and fatigue resistance are higher when 532 nm light with low photobleaching is used in both aqueous solution and living systems. The authors anticipate that their reversible NIR fluorescence photoswitch mediated by DAE can be beneficial for fluorescence imaging in aqueous media requiring accurate detection, such as in the autofluorescence-rich living environment.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Imagen Óptica / Colorantes Fluorescentes Límite: Animals Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Imagen Óptica / Colorantes Fluorescentes Límite: Animals Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article