Microfluidic Seeding of Cells on the Inner Surface of Alginate Hollow Microfibers.
Adv Healthc Mater
; 11(11): e2102701, 2022 06.
Article
en En
| MEDLINE
| ID: mdl-35142451
ABSTRACT
Mimicking microvascular tissue microenvironment in vitro calls for a cytocompatible technique of manufacturing biocompatible hollow microfibers suitable for cell-encapsulation/seeding in and around them. The techniques reported to date either have a limit on the microfiber dimensions or undergo a complex manufacturing process. Here, a microfluidic-based method for cell seeding inside alginate hollow microfibers is designed whereby mouse astrocytes (C8-D1A) are passively seeded on the inner surface of these hollow microfibers. Collagen I and poly-d-lysine, as cell attachment additives, are tested to assess cell adhesion and viability; the results are compared with nonadditive-based hollow microfibers (BARE). The BARE furnishes better cell attachment and higher cell viability immediately after manufacturing, and an increasing trend in the cell viability is observed between Day 0 and Day 2. Swelling analysis using percentage initial weight and width is performed on BARE microfibers furnishing a maximum of 124.1% and 106.1%, respectively. Degradation analysis using weight observed a 62% loss after 3 days, with 46% occurring in the first 12 h. In the frequency sweep test performed, the storage modulus (G') remains comparatively higher than the loss modulus (Gâ³) in the frequency range 0-20 Hz, indicating high elastic behavior of the hollow microfibers.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Microfluídica
/
Alginatos
Límite:
Animals
Idioma:
En
Revista:
Adv Healthc Mater
Año:
2022
Tipo del documento:
Article
País de afiliación:
Estados Unidos