Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells.
Cell Stem Cell
; 29(3): 400-418.e13, 2022 03 03.
Article
en En
| MEDLINE
| ID: mdl-35143761
Totipotent cells have more robust developmental potency than any other cell types, giving rise to both embryonic and extraembryonic tissues. Stable totipotent cell cultures and deciphering the principles of totipotency regulation would be invaluable to understand cell plasticity and lineage segregation in early development. Our approach of remodeling the pericentromeric heterochromatin and re-establishing the totipotency-specific broad H3K4me3 domains promotes the pluri-to-totipotency transition. Our protocol establishes a closer match of mouse 2-cell (2C) embryos than any other 2C-like cells. These totipotent-like stem cells (TLSCs) are stable in culture and possess unique molecular features of the mouse 2C embryo. Functionally, TLSCs are competent for germline transmission and give rise to both embryonic and extraembryonic lineages at high frequency. Therefore, TLSCs represent a highly valuable cell type for studies of totipotency and embryology.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Madre Totipotentes
/
Ensamble y Desensamble de Cromatina
Límite:
Animals
Idioma:
En
Revista:
Cell Stem Cell
Año:
2022
Tipo del documento:
Article
País de afiliación:
China