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[Protective effect of ginsenoside Rg_1 aganist diabetic retinopathy by inhibiting NLRP3 inflammasome in type 2 diabetic mice].
Li, Bin; Zhang, Da-Chuan; Li, Xue-Wang; Dong, Xia-Nan; Li, Wei-Ping; Li, Wei-Zu.
Afiliación
  • Li B; Department of Ophthalmology, the Third People's Hospital of Hefei Hefei 230022, China.
  • Zhang DC; Department of Ophthalmology, the Third People's Hospital of Hefei Hefei 230022, China.
  • Li XW; Department of Pharmacology/Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of Education/Pharmacology 3rd Grade Laboratory of State Administration of Traditional Chinese Medicine, Anhui Medical University Hefei 230032, China.
  • Dong XN; Department of Pharmacology/Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of Education/Pharmacology 3rd Grade Laboratory of State Administration of Traditional Chinese Medicine, Anhui Medical University Hefei 230032, China.
  • Li WP; Department of Pharmacology/Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of Education/Pharmacology 3rd Grade Laboratory of State Administration of Traditional Chinese Medicine, Anhui Medical University Hefei 230032, China.
  • Li WZ; Department of Pharmacology/Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of Education/Pharmacology 3rd Grade Laboratory of State Administration of Traditional Chinese Medicine, Anhui Medical University Hefei 230032, China.
Zhongguo Zhong Yao Za Zhi ; 47(2): 476-483, 2022 Jan.
Article en Zh | MEDLINE | ID: mdl-35178992
ABSTRACT
Ginsenoside Rg_1, one of the main active components of precious traditional Chinese medicine Ginseng Radix et Rhizoma, has the anti-oxidative stress, anti-inflammation, anti-aging, neuroprotection, and other pharmacological effects. Diabetic retinopathy(DR), the most common complication of diabetes, is also the main cause of impaired vision and blindness in the middle-aged and the elderly. The latest research shows that ginsenoside Rg_1 can protect patients against DR, but the protection and the mechanism are rarely studied. This study mainly explored the protective effect of ginsenoside Rg_1 against DR in type 2 diabetic mice and the mechanism. High fat diet(HFD) and streptozotocin(STZ) were used to induce type 2 diabetes in mice, and hematoxylin-eosin(HE) staining was employed to observe pathological changes in the retina of mice. The immunohistochemistry was applied to study the localization and expression of nucleotide-binding oligomerization domain-like receptors 3(NLRP3) and vascular endothelial growth factor(VEGF) in retina, and Western blot was used to detect the expression of nuclear factor-kappa B(NF-κB), p-NF-κB, NLRP3, caspase-1, interleukin-1ß(IL-1ß), transient receptor potential channel protein 6(TRPC6), nuclear factor of activated T-cell 2(NFAT2), and VEGF in retina. The results showed that ginsenoside Rg_1 significantly alleviated the pathological injury of retina in type 2 diabetic mice. Immunohistochemistry results demonstrated that ginsenoside Rg_1 significantly decreased the expression of NLRP3 and VEGF in retinal ganglion cells, middle plexiform layer, and outer plexiform layer in type 2 diabetic mice. According to the Western blot results, ginsenoside Rg_1 significantly lowered the expression of p-NF-κB, NLRP3, caspase-1, IL-1ß, TRPC6, NFAT2, and VEGF in retina of type 2 diabetic mice. These findings suggest that ginsenoside Rg_1 can significantly alleviate DR in type 2 diabetic mice, which may be related to inhibition of NLRP3 inflammasome and VEGF. This study provides experimental evidence for the clinical application of ginsenoside Rg_1 in the treatment of DR.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ginsenósidos / Diabetes Mellitus Experimental / Diabetes Mellitus Tipo 2 / Retinopatía Diabética Límite: Aged / Animals / Humans / Middle aged Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Asunto de la revista: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Año: 2022 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ginsenósidos / Diabetes Mellitus Experimental / Diabetes Mellitus Tipo 2 / Retinopatía Diabética Límite: Aged / Animals / Humans / Middle aged Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Asunto de la revista: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Año: 2022 Tipo del documento: Article País de afiliación: China