Isolation and feeder-free primary culture of four cell types from a single human skin sample.
STAR Protoc
; 3(1): 101172, 2022 03 18.
Article
en En
| MEDLINE
| ID: mdl-35199036
ABSTRACT
Four types of primary cells-dermal fibroblasts, dermal microvascular endothelial cells, epidermal keratinocytes, and epidermal melanocytes-can be isolated simultaneously from a single human skin sample, without the use of xenogeneic murine feeder cells. This protocol describes the procedures for isolation of these cells from adult full-thickness skin obtained from surgical discard tissue. The cells isolated using this protocol contain stem cell populations and are competent to form functional skin tissue in three-dimensional reconstructed skin models. For complete details on the use and execution of this profile, please refer to Supp et al. (2002), Boyce et al. (2015), Boyce et al. (2017a), Boyce et al. (2017b), and Supp et al. (2019).
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Piel
/
Células Endoteliales
Límite:
Animals
/
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2022
Tipo del documento:
Article
País de afiliación:
Estados Unidos