Creating memories: molecular mechanisms of CRISPR adaptation.
Trends Biochem Sci
; 47(6): 464-476, 2022 06.
Article
en En
| MEDLINE
| ID: mdl-35236570
ABSTRACT
Prokaryotes use clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated (Cas) proteins as an adaptive immune system. CRISPR-Cas systems preserve molecular memories of infections by integrating short fragments of foreign nucleic acids as spacers into the host CRISPR array in a process termed 'adaptation'. Functional spacers ensure a robust immune response by Cas effectors, which neutralizes subsequent infection through RNA-guided interference pathways. In this review, we summarize recent discoveries that have advanced our understanding of adaptation, with a focus on how functional spacers are generated and incorporated through many widespread, but type-specific, mechanisms. Finally, we highlight future directions and outstanding questions for a more thorough understanding of CRISPR adaptation.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas Asociadas a CRISPR
Idioma:
En
Revista:
Trends Biochem Sci
Año:
2022
Tipo del documento:
Article