Expression of anti-NbHK single-chain antibody in fusion with NSlmb enhances the resistance to Nosema bombycis in Sf9-III cells.
Bull Entomol Res
; 112(4): 502-508, 2022 Aug.
Article
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| MEDLINE
| ID: mdl-35382911
ABSTRACT
Nosema bombycis is a destructive and specific intracellular parasite of silkworm, which is extremely harmful to the silkworm industry. N. bombycis is considered as a quarantine pathogen of sericulture because of its long incubation period and horizontal and vertical transmission. Herein, two single-chain antibodies targeting N. bombycis hexokinase (NbHK) were cloned and expressed in fusion with the N-terminal of Slmb (a Drosophila melanogaster FBP), which contains the F-box domain. Western blotting demonstrated that Sf9-III cells expressed NSlmb-scFv-7A and NSlmb-scFv-6H, which recognized native NbHK. Subsequently, the NbHK was degraded by host ubiquitination system. When challenged with N. bombycis, the transfected Sf9-III cells exhibited better resistance relative to the controls, demonstrating that NbHK is a prospective target for parasite controls and this approach represents a potential solution for constructing N. bombycis-resistant Bombyx mori.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Bombyx
/
Nosema
Tipo de estudio:
Observational_studies
Límite:
Animals
Idioma:
En
Revista:
Bull Entomol Res
Año:
2022
Tipo del documento:
Article
País de afiliación:
China