Fluorescent cysteine probe based on a signal amplification unit, a catalyzed hairpin assembly reaction and Förster resonance energy transfer.
Methods Appl Fluoresc
; 10(3)2022 Apr 20.
Article
en En
| MEDLINE
| ID: mdl-35442215
ABSTRACT
This work developed a sensitive DNA-based fluorescent probe comprising a cysteine binding unit and a signal amplification unit based on a catalyzed hairpin assembly (CHA) reaction. The cysteine binding unit comprises a homodimer of single-stranded DNA (ssDNA) rich in cytosine and held together by silver ions. In the presence of cysteine, the homodimer is disintegrated because of cysteine-silver binding that liberates the ssDNA, which drives the CHA reaction in the signal amplification unit. Förster resonance energy transfer (FRET) was used to report the generation of the amplified double-stranded DNA (dsDNA) product. Under the optimal conditions, the probe provided a good linearity (100-1200 nM), a good detection limit (47.8 ± 2.7 nM) and quantification limit (159.3 ± 5.3 nM), and a good sensitivity (1.900 ± 0.045µM-1). The probe was then used to detect cysteine in nine real food supplement samples. All results provided good recoveries that are acceptable by the AOAC, indicating that it has potential for practical applications.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Transferencia Resonante de Energía de Fluorescencia
Idioma:
En
Revista:
Methods Appl Fluoresc
Año:
2022
Tipo del documento:
Article
País de afiliación:
Tailandia