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Glyphosate-based herbicide induces long-lasting impairment in neuronal and glial differentiation.
Reis, Luã; Raciti, Marilena; Rodriguez, Patricia González; Joseph, Bertrand; Al Rayyes, Ibrahim; Uhlén, Per; Falk, Anna; da Cunha Lima, Suzana Telles; Ceccatelli, Sandra.
Afiliación
  • Reis L; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
  • Raciti M; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
  • Rodriguez PG; Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
  • Joseph B; Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
  • Al Rayyes I; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
  • Uhlén P; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
  • Falk A; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
  • da Cunha Lima ST; Laboratório de Bioprospecção e Biotecnologia, Instituto de Biologia, Universidade Federal da Bahia (UFBA), Salvador, Brazil.
  • Ceccatelli S; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Environ Toxicol ; 37(8): 2044-2057, 2022 Aug.
Article en En | MEDLINE | ID: mdl-35485992
Glyphosate-based herbicides (GBH) are among the most sold pesticides in the world. There are several formulations based on the active ingredient glyphosate (GLY) used along with other chemicals to improve the absorption and penetration in plants. The final composition of commercial GBH may modify GLY toxicological profile, potentially enhancing its neurotoxic properties. The developing nervous system is particularly susceptible to insults occurring during the early phases of development, and exposure to chemicals in this period may lead to persistent impairments on neurogenesis and differentiation. The aim of this study was to evaluate the long-lasting effects of a sub-cytotoxic concentration, 2.5 parts per million of GBH and GLY, on the differentiation of human neuroepithelial stem cells (NES) derived from induced pluripotent stem cells (iPSC). We treated NES cells with each compound and evaluated the effects on key cellular processes, such as proliferation and differentiation in daughter cells never directly exposed to the toxicants. We found that GBH induced a more immature neuronal profile associated to increased PAX6, NESTIN and DCX expression, and a shift in the differentiation process toward glial cell fate at the expense of mature neurons, as shown by an increase in the glial markers GFAP, GLT1, GLAST and a decrease in MAP2. Such alterations were associated to dysregulation of key genes critically involved in neurogenesis, including PAX6, HES1, HES5, and DDK1. Altogether, the data indicate that subtoxic concentrations of GBH, but not of GLY, induce long-lasting impairments on the differentiation potential of NES cells.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Herbicidas Límite: Humans Idioma: En Revista: Environ Toxicol Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Suecia

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Herbicidas Límite: Humans Idioma: En Revista: Environ Toxicol Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Suecia