Biomolecules capturing live bacteria from clinical samples.
Trends Biochem Sci
; 47(8): 673-688, 2022 08.
Article
en En
| MEDLINE
| ID: mdl-35487808
ABSTRACT
Rapid phenotypic antimicrobial susceptibility testing (AST) requires the enrichment of live bacteria from patient samples, which is particularly challenging in the context of life-threatening bloodstream infections (BSIs) due to low bacterial titers. Over two decades, an extensive array of pathogen-specific biomolecules has been identified to capture live bacteria. The prevailing biomolecules are immune proteins of the complement system, antibodies, aptamers, phage proteins, and antimicrobial peptides. These biomolecules differ by their binder generation technologies and exhibit highly variable specificities, ranging from bacterial strains to most pathogenic bacteria. Here, we summarize how these diverse biomolecules were identified, list examples of successfully reported capture assays, and provide an outlook on the use of nanobodies raised against conserved surface-accessible proteins as promising biomolecules for pathogen capture.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Bacterias
/
Bacteriófagos
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Trends Biochem Sci
Año:
2022
Tipo del documento:
Article
País de afiliación:
Suiza