Engineering a U-box of E3 ligase E4B through yeast surface display-based functional screening generates a variant with enhanced ubiquitin ligase activity.

Park, Seong-Wook; Lee, Da-Som; Kim, Yong-Sung

Park, Seong-Wook; Lee, Da-Som; Kim, Yong-Sung.

Afiliación

Park SW; Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea.
Lee DS; Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea.
Kim YS; Department of Molecular Science and Technology, Ajou University, Suwon, 16499, Republic of Korea; Department of Allergy and Clinical Immunology, Ajou University Medical School, Suwon, Republic of Korea. Electronic address: kimys@ajou.ac.kr.

Biochem Biophys Res Commun ; 612: 147-153, 2022 07 05.

Article en En | MEDLINE | ID: mdl-35525199

Asunto(s)

Saccharomyces cerevisiae; Ubiquitina-Proteína Ligasas; Humanos; Saccharomyces cerevisiae/genética; Saccharomyces cerevisiae/metabolismo; Ubiquitina/metabolismo; Enzimas Ubiquitina-Conjugadoras/metabolismo; Ubiquitina-Proteína Ligasas/metabolismo; Ubiquitinación

Palabras clave

E3 ligase E4B; Functional screening system; U-box; Ubiquitination; Yeast surface display

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Ubiquitina-Proteína Ligasas Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2022 Tipo del documento: Article

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