Multiscale optical and optoacoustic imaging of amyloid-ß deposits in mice.
Nat Biomed Eng
; 6(9): 1031-1044, 2022 09.
Article
en En
| MEDLINE
| ID: mdl-35835994
ABSTRACT
Deposits of amyloid-ß (Aß) in the brains of rodents can be analysed by invasive intravital microscopy on a submillimetre scale, or via whole-brain images from modalities lacking the resolution or molecular specificity to accurately characterize Aß pathologies. Here we show that large-field multifocal illumination fluorescence microscopy and panoramic volumetric multispectral optoacoustic tomography can be combined to longitudinally assess Aß deposits in transgenic mouse models of Alzheimer's disease. We used fluorescent Aß-targeted probes (the luminescent conjugated oligothiophene HS-169 and the oxazine-derivative AOI987) to transcranially detect Aß deposits in the cortex of APP/PS1 and arcAß mice with single-plaque resolution (8 µm) and across the whole brain (including the hippocampus and the thalamus, which are inaccessible by conventional intravital microscopy) at sub-150 µm resolutions. Two-photon microscopy, light-sheet microscopy and immunohistochemistry of brain-tissue sections confirmed the specificity and regional distributions of the deposits. High-resolution multiscale optical and optoacoustic imaging of Aß deposits across the entire brain in rodents thus facilitates the in vivo study of Aß accumulation by brain region and by animal age and strain.
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1
Banco de datos:
MEDLINE
Asunto principal:
Péptidos beta-Amiloides
/
Placa Amiloide
Límite:
Animals
Idioma:
En
Revista:
Nat Biomed Eng
Año:
2022
Tipo del documento:
Article
País de afiliación:
Suiza