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Immunoglobulin G Subclass Response After Chikungunya Virus Infection.
Patil, Harshad P; Gosavi, Mrunal; Kulkarni, Ruta; Mishra, Akhilesh C; Arankalle, Vidya A.
Afiliación
  • Patil HP; Department of Communicable Diseases, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed to be University), Pune, India.
  • Gosavi M; Department of Communicable Diseases, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed to be University), Pune, India.
  • Kulkarni R; Department of Communicable Diseases, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed to be University), Pune, India.
  • Mishra AC; Department of Communicable Diseases, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed to be University), Pune, India.
  • Arankalle VA; Department of Communicable Diseases, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth (Deemed to be University), Pune, India.
Viral Immunol ; 35(6): 437-442, 2022 07.
Article en En | MEDLINE | ID: mdl-35838586
Various vaccines are under development to prevent chikungunya (CHIKV) infection. For the assessment of the CHIKV vaccine-induced antibody response, it is extremely important to understand antibody response after the infection has occurred. Previously, we assessed IgG response in samples from healthy donors using I-CHIKV and found that IgG1 was the predominant subclass induced after CHIKV infection followed by IgG4. However, IgG3 subclass induction is reported in serum samples from patients with acute CHIKV infection. Therefore, in this study, we evaluated serum/plasma from samples of patients with acute CHIKV infection for the presence of IgG and IgG subclasses against I-CHIKV and recombinant E2 protein (rE2). Out of 44 samples that were positive against I-CHIKV, 43 were found reactive against rE2. The positivity of IgG1 either alone or together with other IgG subclasses using I-CHIKV was 89% samples, while 86% samples were positive using rE2. High titers of IgG1 are obtained with I-CHIKV (67%), while raised IgG4 levels are detected using rE2p (72%) in the samples that are positive for both these subclasses. Testing of 22 samples for neutralizing antibodies revealed 100% IgG1 positivity and neutralizing antibodies in 21, 1 sample negative for both. Overall, these data will be useful in assessing IgG subclass-specific CHIKV neutralization and response after CHIKV immunization.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Fiebre Chikungunya Límite: Humans Idioma: En Revista: Viral Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA / VIROLOGIA Año: 2022 Tipo del documento: Article País de afiliación: India

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Fiebre Chikungunya Límite: Humans Idioma: En Revista: Viral Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA / VIROLOGIA Año: 2022 Tipo del documento: Article País de afiliación: India