Multistage adaptive noise reduction technique for optical resolution photoacoustic microscopy.

Photoacoustic microscopy has received great attention due to the benefits of the optical resolution contrast as well as its superior spatial resolution and relatively deep depth. Like other imaging modalities, photoacoustic images suffer from noise, and filtering techniques are required to remove them. To overcome the noise, we proposed a combination of filters, including an adaptive median filter, an effective filter for impulsive noise, and a nonlocal means filter, an effective filter for background noise, for noise removal and image quality enhancement. Our proposed method enhanced the signal-to-noise ratio by 16 dB in an in vivo study compared to the traditional image reconstruction approach and preserved the image detail with minimal blurring, which usually occurs when filtering. These experimental results verified that the proposed adaptive multistage denoising techniques could effectively improve image quality under noisy data acquisition conditions, providing a strong foundation for photoacoustic microscopy with limited laser power.