Selective c-MYC G4 DNA recognition based on a fluorescent light-up probe with disaggregation-induced emission characteristics.
J Mater Chem B
; 10(38): 7772-7779, 2022 10 05.
Article
en En
| MEDLINE
| ID: mdl-36069214
ABSTRACT
The c-MYC promoter is well-known as an important oncogene, the overexpression of which leads to â¼80% of all solid tumors. The four-stranded G4 present in the c-MYC promoter has been shown to play a pivotal role in the regulation of c-MYC transcription. Accordingly, strategies employed for c-MYC G4 DNA sensing have implications for the detection of many human pathologies. However, achieving specificity toward c-MYC G4 over other structurally similar G4s is a challenging task. Here, a supramolecular strategy that relies on the recognition-driven disaggregation of a novel BODIPY probe is outlined. The synthesized probe remained almost non-fluorescent in aqueous media in the aggregation state. Of all the tested G4 and non-G4 DNAs, only c-MYC triggered probe disaggregation and induced a significant increase in fluorescence intensity. The binding details discussed here suggest the basis for the recognition of a particular G4 structure, thus opening up a new way for the design and development of sequence-selective supramolecular G4 probes with desired properties.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
G-Cuádruplex
Límite:
Humans
Idioma:
En
Revista:
J Mater Chem B
Año:
2022
Tipo del documento:
Article