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Detection of alloimmunization in Glanzmann Thrombasthenia and Bernard-Soulier Syndrome: Data from a Brazilian Center.
Gabe, Caroline; Ziza, Karen Chinoca; Durazzo, Natália; Pagani, Flavia M; Oliveira, Valéria Brito; Conrado, Marina-C A V; Dezan, Marcia R; Mendrone, Alfredo; Villaça, Paula Ribeiro; Dinardo, Carla Luana; Rocha, Vanderson.
Afiliación
  • Gabe C; Faculdade de Medicina da Universidade de São Paulo (FM USP), São Paulo, SP, Brazil. Electronic address: caroline.gabe@usp.br.
  • Ziza KC; Faculdade de Medicina da Universidade de São Paulo (FM USP), São Paulo, SP, Brazil.
  • Durazzo N; Instituto de Medicina Tropical da da Universidade de São Paulo (IMT USP), São Paulo, SP, Brazil.
  • Pagani FM; Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, SP, Brazil.
  • Oliveira VB; Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, SP, Brazil.
  • Conrado MAV; Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, SP, Brazil.
  • Dezan MR; Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, SP, Brazil.
  • Mendrone A; Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, SP, Brazil.
  • Villaça PR; Faculdade de Medicina da Universidade de São Paulo (FM USP), São Paulo, SP, Brazil.
  • Dinardo CL; Instituto de Medicina Tropical da da Universidade de São Paulo (IMT USP), São Paulo, SP, Brazil; Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, SP, Brazil.
  • Rocha V; Faculdade de Medicina da Universidade de São Paulo (FM USP), São Paulo, SP, Brazil; Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, SP, Brazil; Churchill Hospital, NHS BT, Oxford University, Oxford, United Kingdom.
Hematol Transfus Cell Ther ; 45 Suppl 2: S101-S107, 2023 Jul.
Article en En | MEDLINE | ID: mdl-36114116
INTRODUCTION: The Glanzmann Thrombasthenia (GT) and Bernard-Soulier Syndrome (BSS) are rare hereditary disorders of platelet function. Their treatment often requires platelet transfusion, which can lead to the development of alloantibodies. OBJECTIVE: In this study, we aim to develop a strategy for alloantibody detection and to describe the frequency of alloimmunization in a patient population from a single center in southeastern Brazil. METHODS: Samples from patients with GT or BSS were tested using the Platelet Immunofluorescence Test (PIFT). If a positive result was obtained, a confirmatory step using the Monoclonal Antibody Immobilization of Platelet Antigens (MAIPA) and Luminex bead-based platelet assay (PAKLx) was executed. MAIN RESULTS: Among 11 patients with GT, we detected the presence of alloantibodies in 5 using PIFT, with confirmation through MAIPA and PAKLx in 2 (1 anti-HLA and 1 anti-HPA), resulting in a frequency of 18.1%. Among 4 patients with BSS, PIFT was positive in 3, with confirmation by MAIPA and PAKLx in 1 (anti-HLA), showing a frequency of 25%. The two patients with anti-HLA antibodies exhibited a panel reactive antibody (PRA-HLA) testing greater than 97%. CONCLUSION: Our study highlights the importance of identifying platelet alloimmunization in this patient population. The proposed algorithm for platelet alloantibodies detection allows resource optimization.
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Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Diagnostic_studies País/Región como asunto: America do sul / Brasil Idioma: En Revista: Hematol Transfus Cell Ther Año: 2023 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Diagnostic_studies País/Región como asunto: America do sul / Brasil Idioma: En Revista: Hematol Transfus Cell Ther Año: 2023 Tipo del documento: Article