Isolation of dermatan sulfate with high heparin cofactor II-mediated thrombin-inhibitory activity from porcine spleen.
Biochim Biophys Acta
; 925(3): 325-31, 1987 Sep 11.
Article
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| MEDLINE
| ID: mdl-3620505
ABSTRACT
We prepared dermatan sulfate specimens from various porcine tissues, and compared their heparin cofactor II-mediated thrombin-inhibitory activities and chemical natures, including disaccharide composition. Electrophoresis of the specimens on cellulose acetate membrane indicated that spleen dermatan sulfate was the most acidic of the dermatan sulfates prepared from the various porcine tissues. Analysis of the disaccharide units of the dermatan sulfate specimens by high-performance liquid chromatography revealed that spleen dermatan sulfate was rich in 4,6-di-O-sulfated N-acetylgalactosamine residues as compared with those of the other tissues. Spleen dermatan sulfate exhibited the highest thrombin-inhibitory activity, which may be related to its high content of the disulfated N-acetylgalactosamine residue.
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Banco de datos:
MEDLINE
Asunto principal:
Bazo
/
Glicoproteínas
/
Trombina
/
Condroitín
/
Dermatán Sulfato
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1987
Tipo del documento:
Article