Boundary-Preserved Deep Denoising of Stochastic Resonance Enhanced Multiphoton Images.
IEEE J Transl Eng Health Med
; 10: 1800812, 2022.
Article
en En
| MEDLINE
| ID: mdl-36304843
ABSTRACT
OBJECTIVE:
With the rapid growth of high-speed deep-tissue imaging in biomedical research, there is an urgent need to develop a robust and effective denoising method to retain morphological features for further texture analysis and segmentation. Conventional denoising filters and models can easily suppress the perturbative noise in high-contrast images; however, for low photon budget multiphoton images, a high detector gain will not only boost the signals but also bring significant background noise. In such a stochastic resonance imaging regime, subthreshold signals may be detectable with the help of noise, meaning that a denoising filter capable of removing noise without sacrificing important cellular features, such as cell boundaries, is desirable.METHOD:
We propose a convolutional neural network-based denoising autoencoder method - a fully convolutional deep denoising autoencoder (DDAE) - to improve the quality of three-photon fluorescence (3PF) and third-harmonic generation (THG) microscopy images.RESULTS:
The average of 200 acquired images of a given location served as the low-noise answer for the DDAE training. Compared with other conventional denoising methods, our DDAE model shows a better signal-to-noise ratio (28.86 and 21.66 for 3PF and THG, respectively), structural similarity (0.89 and 0.70 for 3PF and THG, respectively), and preservation of the nuclear or cellular boundaries (F1-score of 0.662 and 0.736 for 3PF and THG, respectively). It shows that DDAE is a better trade-off approach between structural similarity and preserving signal regions.CONCLUSIONS:
The results of this study validate the effectiveness of the DDAE system in boundary-preserved image denoising. CLINICAL IMPACT The proposed deep denoising system can enhance the quality of microscopic images and effectively support clinical evaluation and assessment.Palabras clave
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1
Banco de datos:
MEDLINE
Asunto principal:
Redes Neurales de la Computación
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Ruido
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
IEEE J Transl Eng Health Med
Año:
2022
Tipo del documento:
Article