Accumulation of DNA damage alters microRNA gene transcription in Arabidopsis thaliana.
BMC Plant Biol
; 22(1): 576, 2022 Dec 12.
Article
en En
| MEDLINE
| ID: mdl-36503409
ABSTRACT
BACKGROUND:
MicroRNAs (miRNAs) and other epigenetic modifications play fundamental roles in all eukaryotic biological processes. DNA damage repair is a key process for maintaining the genomic integrity of different organisms exposed to diverse stresses. However, the reaction of miRNAs in the DNA damage repair process is unclear.RESULTS:
In this study, we found that the simultaneous mutation of zinc finger DNA 3'-phosphoesterase (ZDP) and AP endonuclease 2 (APE2), two genes that play overlapping roles in active DNA demethylation and base excision repair (BER), led to genome-wide alteration of miRNAs. The transcripts of newly transcribed miRNA-encoding genes (MIRs) decreased significantly in zdp/ape2, indicating that the mutation of ZDP and APE2 affected the accumulation of miRNAs at the transcriptional level. In addition, the introduction of base damage with the DNA-alkylating reagent methyl methanesulfonate (MMS) accelerated the reduction of miRNAs in zdp/ape2. Further mutation of FORMAMIDOPYRIMIDINE DNA GLYCOSYLASE (FPG), a bifunctional DNA glycosylase/lyase, rescued the accumulation of miRNAs in zdp/ape2, suggesting that the accumulation of DNA damage repair intermediates induced the transcriptional repression of miRNAs.CONCLUSIONS:
Our investigation indicates that the accumulation of DNA damage repair intermediates inhibit miRNAs accumulation by inhibiting MIR transcriptions.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Arabidopsis
/
Proteínas de Arabidopsis
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MicroARNs
Idioma:
En
Revista:
BMC Plant Biol
Asunto de la revista:
BOTANICA
Año:
2022
Tipo del documento:
Article
País de afiliación:
China