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Aminoclay Nanoparticles Induce Anti-Inflammatory Dendritic Cells to Attenuate LPS-Elicited Pro-Inflammatory Immune Responses.
Park, Hyun Jung; Lee, Sung Won; Song, Jae Geun; Van Kaer, Luc; Cheon, Jae Hee; Lim, Soo-Jeong; Han, Hyo-Kyung; Hong, Seokmann.
Afiliación
  • Park HJ; Department of Integrative Bioscience and Biotechnology, Institute of Anticancer Medicine Development, Sejong University, Neungdong-ro 209, Gwangjin-gu, Seoul 05006, Republic of Korea.
  • Lee SW; Department of Integrative Bioscience and Biotechnology, Institute of Anticancer Medicine Development, Sejong University, Neungdong-ro 209, Gwangjin-gu, Seoul 05006, Republic of Korea.
  • Song JG; College of Pharmacy, Dongguk University-Seoul, Dongguk-ro 32, Ilsan-donggu, Goyang 10326, Republic of Korea.
  • Van Kaer L; Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
  • Cheon JH; Department of Internal Medicine, Institute of Gastroenterology, Yonsei University College of Medicine, Yonsei-ro 50-1, Seodaemun-gu, Seoul 03722, Republic of Korea.
  • Lim SJ; Department of Integrative Bioscience and Biotechnology, Sejong University, Neungdong-ro 209, Gwangjin-gu, Seoul 05006, Republic of Korea.
  • Han HK; College of Pharmacy, Dongguk University-Seoul, Dongguk-ro 32, Ilsan-donggu, Goyang 10326, Republic of Korea.
  • Hong S; Department of Integrative Bioscience and Biotechnology, Institute of Anticancer Medicine Development, Sejong University, Neungdong-ro 209, Gwangjin-gu, Seoul 05006, Republic of Korea.
Molecules ; 27(24)2022 Dec 09.
Article en En | MEDLINE | ID: mdl-36557876
Although 3-aminopropyl functionalized magnesium phyllosilicate nanoparticles (hereafter aminoclay nanoparticles, ACNs) are well-known nanomaterials employed as drug carriers, their effects on immune cells remain unclear. To address this issue, we explored murine dendritic cells (DCs) as these cells belong to the innate arm of the immune system and function as antigen-presenting cells to elicit adaptive immune responses. We examined the in vitro effects of ACNs on DCs isolated from B6 mice. ACN treatment significantly down-regulated the expression of inflammasome-related markers, including NLRP3, caspase-1, and IL1ß. The ACNs-induced anti-inflammatory DC phenotype was further confirmed by down-regulation of the AKT/mTOR/HIF1α signaling pathway. Such anti-inflammatory effects of ACNs on DCs occurred independently of DC subtypes. To document the effects of ACNs on DCs more clearly, we examined their anti-inflammatory effects on lipopolysaccharide (LPS)-activated DCs. As expected, excessive inflammatory responses (increased mitochondrial ROS and Th1-type cytokines such as IL12 and IL1ß) of LPS-activated DCs were dramatically attenuated by ACN treatment. Furthermore, ACNs down-regulated IFNγ production by antigen-specific CD4+ T cells, which is consistent with a reduced inflammatory phenotype of DCs. Overall, our results provide support for employing ACNs as drug delivery materials with therapeutic potential to control inflammatory disorders.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Lipopolisacáridos / Nanopartículas Límite: Animals Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Lipopolisacáridos / Nanopartículas Límite: Animals Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article