Direct observation of motor protein stepping in living cells using MINFLUX.
Science
; 379(6636): 1010-1015, 2023 03 10.
Article
en En
| MEDLINE
| ID: mdl-36893247
ABSTRACT
Dynamic measurements of molecular machines can provide invaluable insights into their mechanism, but these measurements have been challenging in living cells. Here, we developed live-cell tracking of single fluorophores with nanometer spatial and millisecond temporal resolution in two and three dimensions using the recently introduced super-resolution technique MINFLUX. Using this approach, we resolved the precise stepping motion of the motor protein kinesin-1 as it walked on microtubules in living cells. Nanoscopic tracking of motors walking on the microtubules of fixed cells also enabled us to resolve the architecture of the microtubule cytoskeleton with protofilament resolution.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células
/
Cinesinas
/
Microscopía Fluorescente
/
Microtúbulos
Límite:
Humans
Idioma:
En
Revista:
Science
Año:
2023
Tipo del documento:
Article
País de afiliación:
Alemania