Highly sensitive tandem mass spectrometric measurement of serum estradiol without derivatization and pediatric reference intervals in children and adolescents.
Clin Chem Lab Med
; 61(10): 1820-1828, 2023 09 26.
Article
en En
| MEDLINE
| ID: mdl-37036784
ABSTRACT
OBJECTIVES:
Monitoring estradiol (E2) is important for determining the onset of pubertal development as well as in the evaluation of girls with precocious puberty. However, E2 measurement remains an analytical challenge in children, who have lower circulating levels. We developed and evaluated a simple and sensitive LC-MS/MS procedure for serum E2 quantification in pediatric populations and established age- and sex-specific pediatric reference intervals.METHODS:
Residual patient serum samples were used to evaluate the analytical performance of our in-house LC-MS/MS E2 assay. The evaluation included accuracy, precision, linearity, functional sensitivity (LLoQ), and method comparison. Age- and sex-specific pediatric E2 reference intervals were also established from a cohort of 405 healthy children (birth to 18 years) recruited with informed consent. Age- and sex-specific differences were assessed, and outliers were removed. Reference intervals were established using the robust method.RESULTS:
The assay imprecision was <5.3â¯%. Assay linearity ranged from 13.7 to 1923.3â¯pmol/L. The LLoQ corresponding to a CV of 20â¯% was determined to be 8.9â¯pmol/L. Bland-Altman analysis revealed a mean bias of 29.3â¯pmol/L or 9.1â¯% between our LC-MS/MS E2 assay and an external reference laboratory measuring E2 by LC-MS/MS.CONCLUSIONS:
Our LC-MS/MS E2 assay shows acceptable accuracy, precision, functional sensitivity (LLoQ), and linearity for E2 quantification. Our LC-MS/MS E2 assay also showed good agreement with an external reference laboratory measuring E2 by LC-MS/MS. In addition, using CALIPER samples, we established robust age- and sex-specific pediatric E2 reference intervals to improve accuracy of test result interpretation and clinical decision making.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Estrona
/
Espectrometría de Masas en Tándem
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Adolescent
/
Child
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Clin Chem Lab Med
Asunto de la revista:
QUIMICA CLINICA
/
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2023
Tipo del documento:
Article
País de afiliación:
Canadá