ECL sensor for selective determination of citrate ions as a prostate cancer biomarker using polymer of intrinsic microporosity-1 nanoparticles/nitrogen-doped carbon quantum dots.
Anal Bioanal Chem
; 415(14): 2727-2736, 2023 Jun.
Article
en En
| MEDLINE
| ID: mdl-37042993
ABSTRACT
Urine citrate analysis is relevant in the screening and monitoring of patients with prostate cancer and calcium nephrolithiasis. A sensitive, fast, easy, and low-maintenance electrochemiluminescence (ECL) method with conductivity detection for the analysis of citrate in urine is developed and validated by employing polymer of intrinsic microporosity-1 nanoparticles/nitrogen-doped carbon quantum dots (nano-PIM-1/N-CQDs). Using optimum conditions, the sensor was applied in ECL experiments in the presence of different concentrations of citrate ions. The ECL signals were quenched gradually by the increasing citrate concentration. The linear range of the relationship between the logarithm of the citrate concentration and ΔECL (ECL of blank - ECL of sample) was obtained between 1.0 × 10-7 M and 5.0 × 10-4 M. The limit of detection (LOD) was calculated to be 2.2 × 10-8 M (S/N = 3). The sensor was successfully applied in real samples such as human serum and patient urine.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de la Próstata
/
Puntos Cuánticos
/
Nanopartículas
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
/
Male
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2023
Tipo del documento:
Article
País de afiliación:
Irán