Plasmonic genosensor for detecting hazelnut Cor a 14-encoding gene for food allergen monitoring.
Anal Chim Acta
; 1259: 341168, 2023 Jun 08.
Article
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| MEDLINE
| ID: mdl-37100473
ABSTRACT
A plasmonic nanostructure was constructed as a biorecognition element coupled to an optical sensing platform in sandwich format, targeting the hazelnut Cor a 14 allergen-encoding gene. The analytical performance of the genosensor presented a linear dynamic range between 100 amol L-1 and 1 nmol L-1, a limit of detection (LOD) < 19.9 amol L-1, and a sensitivity of 13.4 ± 0.6 m°. The genosensor was successfully hybridized with hazelnut PCR products, tested with model foods, and further validated by real-time PCR. It reached a LOD <0.001% (10 mg kg-1) of hazelnut in wheat material (corresponding to 1.6 mg kg-1 of protein) and a sensitivity of -17.2 ± 0.5 m° for a linear range of 0.001%-1%. Herein, a new genosensing approach is proposed as a highly sensitive and specific alternative tool with potential application in monitoring hazelnut as an allergenic food, protecting the health of sensitized/allergic individuals.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Corylus
/
Hipersensibilidad a los Alimentos
Límite:
Humans
Idioma:
En
Revista:
Anal Chim Acta
Año:
2023
Tipo del documento:
Article
País de afiliación:
Portugal