Novel human STING activation by hydrated-prenylated xanthones from Garcinia cowa.
J Pharm Pharmacol
; 75(8): 1058-1065, 2023 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-37307431
ABSTRACT
OBJECTIVES:
We investigate the anticancer activity and human stimulator of interferon genes pathway activation by a new hydrated-prenylated tetraoxygenated xanthone, garcicowanone I (1) and two known xanthones (2 and 3) that were isolated from the root bark of Garcinia cowa Roxb. ex Choisy.METHODS:
The anticancer activity of each compound was evaluated by sulforhodamine B assay in immortalized cancer cell lines. Stimulator of interferon genes pathway activation was assessed by western blot analysis using human THP-1-derived macrophages. The production of pro-inflammatory cytokines from these macrophages was also evaluated via enzyme-linked immunosorbent assay. KEYFINDINGS:
Both compounds 1 and 3 displayed moderate inhibitory effects on the cancer cells, including a cisplatin-resistant cell line, with IC50 values in the range of 10-20 µM. All three xanthones activated the stimulator of interferon genes, as evidenced by phosphorylation of tank-binding kinase 1, the stimulator of interferon genes protein and interferon regulatory factor 3. Furthermore, treatment of these macrophages with compounds 1-3 led to the production of pro-inflammatory cytokines, including interleukin 6, tumour necrosis factor α and interleukin 1ß.CONCLUSIONS:
In conclusion, the isolated xanthones, including the novel garcicowanone I, displayed promising anticancer and immunomodulatory activity that warrants further research.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Garcinia
/
Xantonas
Límite:
Humans
Idioma:
En
Revista:
J Pharm Pharmacol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Vietnam