E. coli "Stablelabel" S30 lysate for optimized cell-free NMR sample preparation.

Levin, Roman; Löhr, Frank; Karakoc, Betül; Lichtenecker, Roman; Dötsch, Volker; Bernhard, Frank

Levin, Roman; Löhr, Frank; Karakoc, Betül; Lichtenecker, Roman; Dötsch, Volker; Bernhard, Frank.

Afiliación

Levin R; Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438, Frankfurt, Germany.
Löhr F; Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438, Frankfurt, Germany.
Karakoc B; Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438, Frankfurt, Germany.
Lichtenecker R; Institute of Organic Chemistry, University of Vienna, 1090, Vienna, Austria.
Dötsch V; MAG-LAB, 1030, Vienna, Austria.
Bernhard F; Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438, Frankfurt, Germany.

J Biomol NMR ; 77(4): 131-147, 2023 Aug.

Article en En | MEDLINE | ID: mdl-37311907

Asunto(s)

Aminoácidos; Escherichia coli; Escherichia coli/metabolismo; Resonancia Magnética Nuclear Biomolecular/métodos; Aminoácidos/química; Proteínas/química; Biosíntesis de Proteínas; Marcaje Isotópico/métodos; Sistema Libre de Células/metabolismo

Palabras clave

Cell-free expression; Metabolic engineering; NMR of membrane proteins; Protein labeling; S30 lysate; Stable isotope scrambling

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Escherichia coli / Aminoácidos Idioma: En Revista: J Biomol NMR Asunto de la revista: BIOLOGIA MOLECULAR / DIAGNOSTICO POR IMAGEM / MEDICINA NUCLEAR Año: 2023 Tipo del documento: Article País de afiliación: Alemania

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