Clinical Implications of Discrepancy between One-Stage Clotting and Chromogenic Factor IX Activity in Hemophilia B.
Thromb Haemost
; 124(1): 32-39, 2024 Jan.
Article
en En
| MEDLINE
| ID: mdl-37494968
ABSTRACT
BACKGROUND:
Discrepancy in factor IX activity (FIXC) between one-stage assay (OSA) and chromogenic substrate assay (CSA) in patients with hemophilia B (PwHB) introduces challenges for clinical management.AIM:
To study the differences in FIXC using OSA and CSA in moderate and mild hemophilia B (HB), their impact on classification of severity, and correlation with genotype.METHODS:
Single-center study including 21 genotyped and clinically characterized PwHB. FIXC by OSA was measured using ActinFSL (Siemens) and CSA by Biophen (Hyphen). In addition, in vitro experiments with wild-type FIX were performed. Reproducibility of CSA was assessed between three European coagulation laboratories.RESULTS:
FIXC by CSA was consistently lower than by OSA, with 10/17 PwHB having a more severe hemophilia type by CSA. OSA displayed a more accurate description of the clinical bleeding severity, compared with CSA. A twofold difference between OSACSA FIXC was present in 12/17 PwHB; all patients had genetic missense variants in the FIX serine protease domain. Discrepancy was also observed with diluted normal plasma, most significant for values below 0.10 IU/mL. Assessment of samples with low FIXC showed excellent reproducibility of the CSA results between the laboratories.CONCLUSION:
FIXC was consistently higher by OSA compared with the CSA. Assessing FIXC by CSA alone would have led to diagnosis of a more severe hemophilia type in a significant proportion of patients. Our study suggests using both OSA and CSA FIXC together with genotyping to classify HB severity and provide essential information for clinical management.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Hemofilia B
/
Hemofilia A
Límite:
Humans
Idioma:
En
Revista:
Thromb Haemost
Año:
2024
Tipo del documento:
Article
País de afiliación:
Suecia