Generation of two human iPSC lines from dermal fibroblasts of adult- and juvenile-onset Huntington's disease patients and two healthy donors.
Stem Cell Res
; 71: 103194, 2023 09.
Article
en En
| MEDLINE
| ID: mdl-37651831
ABSTRACT
Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by a mutation in the HTT gene. To generate human-induced pluripotent stem cells (hiPSCs), we used dermal fibroblasts from 1 healthy adult control (K-Pic2), 1 HD manifest patient (M-T2), 1 healthy juvenile control (jK-N1), and 1 juvenile HD patient (jHD-V1). HD stage of patients was assessed by neurological tests and donors were without comorbidities and were non-smokers. Characterization showed that the obtained hiPSCs have the same number of CAG repeats as the parental fibroblast lines, express pluripotency markers and have the ability to differentiate into all 3 germ layers.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Artrogriposis
/
Enfermedad de Huntington
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Enfermedades Neurodegenerativas
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Células Madre Pluripotentes Inducidas
Límite:
Adult
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Humans
Idioma:
En
Revista:
Stem Cell Res
Año:
2023
Tipo del documento:
Article
País de afiliación:
Polonia