Performance evaluation of two multiplex qualitative RT-PCR assays for detection of respiratory infection in paediatric population.
Malays J Pathol
; 45(2): 215-227, 2023 Aug.
Article
en En
| MEDLINE
| ID: mdl-37658531
ABSTRACT
INTRODUCTION:
Acute respiratory infection (ARI) contributes to significant mortality and morbidity worldwide and is usually caused by a wide range of respiratory pathogens. This study aims to describe the performance of QIAstat-Dx® Respiratory Panel V2 (RP) and RespiFinder® 2SMART assays for respiratory pathogens detection. MATERIALS ANDMETHODS:
A total of 110 nasopharyngeal swabs (NPS) were collected from children aged one month to 12 years old who were admitted with ARI in UKMMC during a one-year period. The two qPCR assays were conducted in parallel.RESULTS:
Ninety-seven samples (88.2%) were positive by QIAstat-Dx RP and 86 (78.2%) by RespiFinder assay. The overall agreement on both assays was substantial (kappa value 0.769) with excellent concordance rate of 96.95%. Using both assays, hRV/EV, INF A/H1N1 and RSV were the most common pathogens detected. Influenza A/H1N1 infection was significantly seen higher in older children (age group > 60 months old) (53.3%, p-value < 0.05). Meanwhile, RSV and hRV/EV infection were seen among below one-year-old children. Co-infections by two to four pathogens were detected in 17 (17.5%) samples by QIAstat-Dx RP and 12 (14%) samples by RespiFinder, mainly involving hRV/EV. Bacterial detection was observed only in 5 (4.5%) and 6 (5.4%) samples by QIAstat-Dx RP and RespiFinder, respectively, with Mycoplasma pneumoniae the most common detected.CONCLUSION:
The overall performance of the two qPCR assays was comparable and showed excellent agreement. Both detected various clinically important respiratory pathogens in a single test with simultaneous multiple infection detection. The use of qPCR as a routine diagnostic test can improve diagnosis and management.
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Banco de datos:
MEDLINE
Asunto principal:
Infecciones del Sistema Respiratorio
/
Subtipo H1N1 del Virus de la Influenza A
/
Coinfección
Tipo de estudio:
Diagnostic_studies
/
Qualitative_research
Límite:
Child
/
Child, preschool
/
Humans
/
Infant
Idioma:
En
Revista:
Malays J Pathol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Malasia