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The novel developed microsatellite markers revealed potential hybridization among Cymbidium species and the interspecies sub-division of C. goeringii and C. ensifolium.
Ning, Hui-Juan; Gui, Fang-Fang; Tian, En-Wei; Yang, Li-Yuan.
Afiliación
  • Ning HJ; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, School of Landscape and Architecture, Zhejiang A&F University, Hangzhou, 311300, Zhejiang, China.
  • Gui FF; Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, School of Landscape and Architecture, Zhejiang A&F University, Hangzhou, 311300, Zhejiang, China.
  • Tian EW; School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 515005, China. tianenwei@126.com.
  • Yang LY; Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, Guangzhou, 510515, China. tianenwei@126.com.
BMC Plant Biol ; 23(1): 492, 2023 Oct 13.
Article en En | MEDLINE | ID: mdl-37833649
ABSTRACT

BACKGROUND:

Orchids (Cymbidium spp.) exhibit significant variations in floral morphology, pollinator relations, and ecological habitats. Due to their exceptional economic and ornamental value, Cymbidium spp. have been commercially cultivated for centuries. SSR markers are extensively used genetic tools for biology identification and population genetics analysis.

RESULT:

In this study, nine polymorphic EST-SSR loci were isolated from Cymbidium goeringii using RNA-Seq technology. All nine SSR loci showed transferability in seven other congeneric species, including 51 cultivars. The novel SSR markers detected inter-species gene flow among the Cymbidium species and intra-species sub-division of C. goeringii and C. ensifolium, as revealed by neighborhood-joining and Structure clustering analyses.

CONCLUSION:

In this study, we developed nine microsatellites using RNA-Seq technology. These SSR markers aided in detecting potential gene flow among Cymbidium species and identified the intra-species sub-division of C. goeringii and C. ensifolium.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Orchidaceae / Genética de Población Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Orchidaceae / Genética de Población Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: China