The toxic profile of tramadol combined with nicotine on the liver and testicles: evidence from endoplasmic reticulum stress.
Mol Biol Rep
; 50(12): 9887-9895, 2023 Dec.
Article
en En
| MEDLINE
| ID: mdl-37864661
ABSTRACT
BACKGROUND:
Tramadol is one of the most commonly abused substances in the Middle East. Furthermore, smoking is extremely common among the population.METHODS:
An experimental study was performed on Sprague-Dawley rats to explore the effects of both nicotine and tramadol on the liver and testes. The tramadol was administered at 10 and 20 mg/kg, respectively, while the nicotine was administered at 125 mg/kg. Histological examination and androgen receptor ELISA assay showed mild effects on the liver and proofed safety on the testis. Western blot analysis of BIP (immunoglobulin heavy-chain binding protein) and CHOP (CCAAT-enhancer-binding protein homologous protein) revealed that fewer problems were induced by adding nicotine to tramadol. Autophagy marker LCIII and apoptosis marker caspase-8 showed similar effects to CHOP and BIP on liver samples. The real-time PCR of BIP expression showed similar but not identical results.CONCLUSIONS:
The results showed mild endoplasmic reticulum stress, autophagy, and apoptosis in the liver samples. Histological examination revealed stable spermatogenesis with average androgen receptor blood levels in the different groups.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Testículo
/
Tramadol
Límite:
Animals
Idioma:
En
Revista:
Mol Biol Rep
Año:
2023
Tipo del documento:
Article
País de afiliación:
Jordania