Human epithelial lung cell toxicity assessment of collected graphite particles from an iron casting industry (in vitro study).
Int J Environ Health Res
; 34(9): 3126-3135, 2024 Sep.
Article
en En
| MEDLINE
| ID: mdl-38146704
ABSTRACT
Workers in the iron casting industries are exposed to various chemicals, especially graphite in furnace process. This study aims to investigate the toxic effects of graphite particles on human lung cells. Particle characteristics were confirmed by electron microscope and light scattering. Cell viability and oxidative stress markers were measured. The expression of oxidative repair genes, namely OGG1, MTH1, and ITPA, was evaluated. The average particle size was determined to be 172.1 ± 11.96 nm. The median inhibition concentration (IC50) of graphite particles was 46.75 µg/mL. Notably, 25 and 50 µg/mL concentrations resulted in significant GSH depletion and MDA production. The high concentration of graphite particles (200 µg/mL) led to OGG1 suppression and increased MTH1 expression. Based on these findings, graphite exposure may induce toxicity in human lung cells by increasing oxidative stress. Further research is necessary to fully understand the mechanisms underlying graphite toxicity.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Tamaño de la Partícula
/
Supervivencia Celular
/
Estrés Oxidativo
/
Células Epiteliales
/
Grafito
Límite:
Humans
Idioma:
En
Revista:
Int J Environ Health Res
Asunto de la revista:
SAUDE AMBIENTAL
Año:
2024
Tipo del documento:
Article
País de afiliación:
Irán