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Limits of Non-invasive Enzymatic Activation by Local Temperature Control.
Vyas, Anisha; Petrásek, Zdenek; Nidetzky, Bernd.
Afiliación
  • Vyas A; Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, Graz, A-8010, Austria.
  • Petrásek Z; Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, Graz, A-8010, Austria.
  • Nidetzky B; Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, Graz, A-8010, Austria.
Small ; 20(30): e2312220, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38344893
ABSTRACT
Enzymatic activity depends on and can therefore be regulated by temperature. Selective modulation of the activity of different enzymes in one reaction pot would require temperature control local to each type of enzyme. It has been suggested previously that immobilization of enzyme on magnetic nanoparticles and exposing them to alternating magnetic field can enhance the reaction rate. This enhancement has been explained as being mediated by temperature increase caused by dissipation of the absorbed field energy in the form of heat. However, the possibility of spatially limiting this temperature increase on the microscale has been questioned. Here, it is investigated whether an activity enhancement of the enzyme sucrose phosphorylase immobilized on magnetic beads can be achieved, how this effect is related to the increase in temperature, and whether temperature differences within one reaction pot could be generated in this way. It is found that alternating magnetic field stimulation leads to increased enzymatic activity fully attributable to the increase of bulk temperature. Both theoretical analysis and experimental data indicate that no local heating near the particle surface takes place. It is further concluded that relevant increase of surface temperature can be obtained only with macroscopic, millimeter-sized, magnetic particles.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Temperatura / Activación Enzimática Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Austria

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Temperatura / Activación Enzimática Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Austria