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The Photoprotective Behavior of a Motile Benthic Diatom as Elucidated from the Interplay Between Cell Motility and Physiological Responses to a Light Microgradient Using a Novel Experimental Setup.
Morelle, Jérôme; Bastos, Alexandra; Frankenbach, Silja; Frommlet, Jörg C; Campbell, Douglas A; Lavaud, Johann; Serôdio, João.
Afiliación
  • Morelle J; CESAM-Centre for Environmental and Marine Studies and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal. jerome.morelle@ua.pt.
  • Bastos A; CESAM-Centre for Environmental and Marine Studies and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.
  • Frankenbach S; CESAM-Centre for Environmental and Marine Studies and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.
  • Frommlet JC; CESAM-Centre for Environmental and Marine Studies and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.
  • Campbell DA; Biology Department, Mount Allison University, Sackville, NB, Canada.
  • Lavaud J; LEMAR-Laboratory of Marine Environmental Sciences, UMR 6539 CNRS, Univ Brest, Ifremer, IRD, Institut Universitaire Européen de La Mer, Technopôle Brest-Iroise, Plouzané, France.
  • Serôdio J; CESAM-Centre for Environmental and Marine Studies and Department of Biology, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.
Microb Ecol ; 87(1): 40, 2024 Feb 13.
Article en En | MEDLINE | ID: mdl-38351424
ABSTRACT
It has long been hypothesized that benthic motile pennate diatoms use phototaxis to optimize photosynthesis and minimize photoinhibitory damage by adjusting their position within vertical light gradients in coastal benthic sediments. However, experimental evidence to test this hypothesis remains inconclusive, mainly due to methodological difficulties in studying cell behavior and photosynthesis over realistic spatial microscale gradients of irradiance and cell position. In this study, a novel experimental approach was developed and used to test the hypothesis of photosynthesis optimization through motility, based on the combination of single-cell in vivo chlorophyll fluorometry and microfluidic chips. The approach allows the concurrent study of behavior and photosynthetic activity of individual cells of the epipelic diatom species Craspedostauros britannicus exposed to a light microgradient of realistic dimensions, simulating the irradiance and distance scales of light microgradients in benthic sediments. Following exposure to light, (i) cells explored their light environment before initiating light-directed motility; (ii) cells used motility to lower their light dose, when exposed to the highest light intensities; and (iii) motility was combined with reversible non-photochemical quenching, to allow cells to avoid photoinhibition. The results of this proof-of-concept study not only strongly support the photoprotective nature of photobehavior in the studied species but also revealed considerable variability in how individual cells reacted to a light microgradient. The experimental setup can be readily applied to study motility and photosynthetic light responses of other diatom species or natural assemblages, as well as other photoautotrophic motile microorganisms, broadening the toolset for experimental microbial ecology research.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Diatomeas Idioma: En Revista: Microb Ecol Año: 2024 Tipo del documento: Article País de afiliación: Portugal

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Diatomeas Idioma: En Revista: Microb Ecol Año: 2024 Tipo del documento: Article País de afiliación: Portugal