Cytogenetic Bioindication in Root Meristems for Vitality Assessment of Trees.
Methods Mol Biol
; 2787: 95-103, 2024.
Article
en En
| MEDLINE
| ID: mdl-38656484
ABSTRACT
Our method describes how to collect forest tree root tips in the field, to store them for transfer to the lab, to pretreat root tips in order to arrest cells in metaphase, fix root tips to preserve specific morphological organizations, to stain fixed root tips by Feulgen's Reaction in order to increase contrast, and to prepare the root meristem for analyzing mitotic stages and chromosomal aberrations via light microscopy. We further describe how to classify chromosomal abnormalities and quantify them via aberration indices.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Árboles
/
Meristema
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2024
Tipo del documento:
Article
País de afiliación:
Austria